Fig 1: APH-induced iAG display the molecular signature of native AG neurons(A–X) Double immunostaining of APH-induced iAG neurons with the indicated antibodies including GFP, Tuj1, Map2, NF200, Synapsin, VIP, NeuN, Peripherin, Vamp1, GABA, GAD65, VGlut2, NPY, SOM, TH, DBH, Vmat2, VAChT, ChAT, Nicotina3R, Nicotinß4R, TrkA, and TrkC. The DAPI nuclear counterlabeling is in blue. A-E: Tau-GFP MEFs; F-V: wild-type MEFs.(Y and Z) qRT-PCR analyses of expression of the indicated genes in MEFs infected with GFP and APH viruses. These genes represent general as well as autonomic subtype-specific neuronal markers. Data are presented as means ± SEM (n = 3).(A') Quantification of Tuj1-positive neurons that express GABA, TH, and ChAT in MEFs infected with the APH viruses. Data are presented as means ± SEM (n = 3).(B') qRT-PCR analysis of the expression of Tubb3 (Tuj1), Map2, TH, and DBH in MEFs infected with GFP, A, AP(2a), AP(2b), AH, AP(2a)H, or AP(2b)H viruses. All qRT-PCR expression levels are relative to that induced by GFP and data are presented as means ± SEM (n = 3). Scale bars: A-D, F, G, I, N, P, Q, 40 µm; E, H, J, K-M, O, R-X, 20 µm. *p < 0.05, &p < 0.01, #p < 0.001 vs GFP group.